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Journal / Developmental Biology / 树突棘从锥体神经元的三维定量从人类诱导多能干细胞衍生…
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树突棘从锥体神经元的三维定量从人类诱导多能干细胞衍生
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树突棘从锥体神经元的三维定量从人类诱导多能干细胞衍生

Article DOI: 10.3791/53197-v • 10:18 min • October 10th, 2015
October 10th, 2015 •
1,2,3, 4, 1,2,3, 5, 1,2,3, 1,2,3
1Human Genetics and Cognitive Functions, Institut Pasteur, 2CNRS URA 2182 'Genes, synapses and cognition', Institut Pasteur, 3Human Genetics and Cognitive Functions, Université Paris Diderot, Sorbonne Paris Cité, 4Plateforme d' Imagerie Dynamique, Imagopole, CiTech, Institut Pasteur, 5Neuroplasticity and Therapeutics, CECS, I-STEM, AFM, Evry

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锥体神经元的树突棘是哺乳动物大脑皮质最兴奋性突触的部位。此方法描述在从诱导的多能干细胞衍生的人皮质锥体谷氨酸能神经元脊柱形态的三维定量分析。

Tags

发育生物学，第104，人类源自iPSC的神经元，锥体细胞，树突棘成熟，脊柱成像，三维量化，共焦显微镜
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