Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

Timothy J. Cashman; Rebecca Josowitz; Bruce D. Gelb; Ronald A. Li; Nicole C. Dubois; Kevin D. Costa

doi:10.3791/53447

Journal

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생체공학

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정의 인간 공학 심장 조직의 건설 심장 세포 치료의 메커니즘을 연구하는

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

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JoVE 신문 생체공학

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

정의 인간 공학 심장 조직의 건설 심장 세포 치료의 메커니즘을 연구하는

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10,363 Views

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11:51 min

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March 01, 2016

DOI:

10.3791/53447-v

DOI:

10.3791/53447-v

•

11:51 min

March 01, 2016

•

10337 Views

Timothy J. Cashman¹, Rebecca Josowitz², Bruce D. Gelb², Ronald A. Li^1,3, Nicole C. Dubois², Kevin D. Costa¹

¹Cardiovascular Research Center,Icahn School of Medicine at Mount Sinai ²The Mindich Child Health and Development Institute,Icahn School of Medicine at Mount Sinai ³Stem Cell & Regenerative Medicine Consortium, LKS Faculty of Medicine,University of Hong Kong

Summary

This manuscript describes the creation of defined engineered cardiac tissues using surface marker expression and cell sorting. The defined tissues can then be used in a multi-tissue bioreactor to investigate mechanisms of cardiac cell therapy in order to provide a functional, yet controlled, model system of the human heart.