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Journal / Genetics / siRNA-Transfektion und EMSA-Analysen auf frisch…
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Genetics

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siRNA-Transfektion und EMSA-Analysen auf frisch isolierten menschlichen Villöse Cytotrophoblasten
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siRNA-Transfektion und EMSA-Analysen auf frisch isolierten menschlichen Villöse Cytotrophoblasten

Article DOI: 10.3791/53995-v • 09:57 min • September 20th, 2016
September 20th, 2016 •
1, 2, 3, 1
1Department of Biological Sciences, University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics, McGill University, 3Department of Clinical Sciences, University of Montreal

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Dieses Protokoll beschreibt ein Verfahren zur siRNA effizient transfizieren in frisch isolierten menschlichen villöse Zytotrophoblasten Verwendung Mikroporation und Identifizieren von DNA-Protein-Komplexe in diesen Zellen. Transfizierte Zellen können durch Western-Blot überwacht werden und EMSA-Analysen während der 4-tägigen Kulturzeit.

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Genetik Heft 115 Molecular Biology Ausgabe Menschliches villous Cytotrophoblasten Syncytin-2 siRNA Mikroporation Lipid-basierte Transfektion elektrophoretische Mobilitäts-Shift-Assay
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