Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / siRNA Transfektion och EMSA Analyser av nyligen…
JoVE Journal
Genetics

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Genetics
siRNA Transfektion och EMSA Analyser av nyligen isolerade mänskliga villös cytotrofoblaster
The TreadWheel: Interval Training Protocol for Gently Induced Exercise in Drosophila melanogaster
Journal (Genetics)
The TreadWheel: Interval Training Protocol for Gently Induced Exercise in Drosophila melanogaster
An Effective Inoculation Method for Phytophthora capsici on Black Pepper Plants
Journal (Genetics)
An Effective Inoculation Method for Phytophthora capsici on Black Pepper Plants
 
Click here for the English version

siRNA Transfektion och EMSA Analyser av nyligen isolerade mänskliga villös cytotrofoblaster

Article DOI: 10.3791/53995-v • 09:57 min • September 20th, 2016
September 20th, 2016 •
1, 2, 3, 1
1Department of Biological Sciences, University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics, McGill University, 3Department of Clinical Sciences, University of Montreal

챕터

요약
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Detta protokoll beskriver en metod för att effektivt transfektera siRNA i färsk isolerade humana villösa cytotrofoblaster genom att använda mikroporation och identifiera DNA-protein-komplex i dessa celler. Transfekterade celler kan övervakas med Western blot och EMSA-analyser under 4-dagars odling tid.

Tags

Genetik molekylärbiologi mänskliga villösa cytotrofoblaster Syncytin-2 siRNA mikroporation Lipid-baserade transfektion Elektro Mobility Shift Assay
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter