Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Mikko Juusola; An Dau; Lei Zheng; Diana Rien

doi:10.3791/54142

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신경과학

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의 기록 세포 내 전압 응답에 대한 전기 생리 방법 초파리 빛 자극에 광수와의 interneurons 생체</em

Electrophysiological Method for Recording Intracellular Voltage Responses of <em>Drosophila</em> Photoreceptors and Interneurons to Light Stimuli <em>In Vivo</em>

JoVE 신문
신경과학

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JoVE 신문 신경과학

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

의 기록 세포 내 전압 응답에 대한 전기 생리 방법 초파리 빛 자극에 광수와의 interneurons 생체</em

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19,534 Views

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11:42 min

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June 19, 2016

DOI:

10.3791/54142-v

DOI:

10.3791/54142-v

•

11:42 min

June 19, 2016

•

19496 Views

Mikko Juusola^1,2, An Dau², Lei Zheng¹, Diana Rien^1,2

¹National Key Laboratory of Cognitive Neuroscience and Learning,Beijing Normal University ²Department of Biomedical Science,The University of Sheffield

Summary

Sharp microelectrodes enable accurate electrophysiological characterization of photoreceptor and visual interneuron output in living Drosophila. Here we show how to use this method to record high-quality voltage responses of individual cells to controlled light stimulation. This method is ideal for studying neural information processing in insect compound eyes.