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Journal / Immunology and Infection / Generieren
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Immunology and Infection

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Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
Journal (Immunology and Infection)
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension
Journal (Immunology and Infection)
Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension
 
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Generieren

Article DOI: 10.3791/54697-v • 08:48 min • October 25th, 2016
October 25th, 2016 •
1,2, *2, *2, 2, 1,2, 1,2, 2, 2, 1,2, 1,2
1Department of Immunology, University of Toronto, 2Princess Margaret Cancer Centre, University Health Network
* These authors contributed equally

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Wir beschreiben hier ein neues Verfahren antigen-spezifische T-Zell-Rezeptoren (TCRs) durch Paarung der TCR & agr; oder TCR & bgr; TCR eines bestehenden, besitzen die Antigen-Spezifität von Interesse, mit komplementären hemichain der peripheren T-Zell-Rezeptor-Repertoire zu erzeugen. Die de novo erzeugt TZR behalten Antigen-Spezifität mit Affinität zu variieren.

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Immunology Ausgabe 116 T-Zellen Antigen-präsentierende Zelle T-Zell-Rezeptor hemichain retrovirale Transduktion Antigen-Spezifität menschlichen Clonierung
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