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Journal / Immunology and Infection / 生成
JoVE Journal
Immunology and Infection

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Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Journal (Immunology and Infection)
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Partial Heterotopic Hindlimb Transplantation Model in Rats
Journal (Immunology and Infection)
Partial Heterotopic Hindlimb Transplantation Model in Rats
 
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生成

Article DOI: 10.3791/54697-v • 08:48 min • October 25th, 2016
October 25th, 2016 •
1,2, *2, *2, 2, 1,2, 1,2, 2, 2, 1,2, 1,2
1Department of Immunology, University of Toronto, 2Princess Margaret Cancer Centre, University Health Network
* These authors contributed equally

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本明細書において、我々は、末梢T細胞受容体レパートリーの相補hemichainと、関心対象の抗原特異性を有する、既存のTCRのTCRα又はTCRβをペアリングすることにより、抗原特異的T細胞受容体（TCR）を生成する新規な方法を記載します。 デノボ生成されたTCRは、親和性を変化させて、抗原特異性を保持します。

Tags

免疫号116、T細胞、抗原提示細胞、T細胞受容体、hemichain、レトロウイルス形質導入、抗原特異性、ヒト、クローニング
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