Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Hurtig neuronal differentiering af induceret…
JoVE Journal
Developmental Biology

이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.

JoVE Journal Developmental Biology
Hurtig neuronal differentiering af induceret pluripotente stamceller til måling Network Aktivitet på Micro-elektrode Arrays
Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro
Journal (Developmental Biology)
Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro
Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
Journal (Developmental Biology)
Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
 
Click here for the English version

Hurtig neuronal differentiering af induceret pluripotente stamceller til måling Network Aktivitet på Micro-elektrode Arrays

Article DOI: 10.3791/54900-v • 09:20 min • January 8th, 2017
January 8th, 2017 •
*1,2, *3, 2,3, 3, 1,2, 1,2, 1,2, 2,3,4, 1,2,3
1Department of Cognitive Neurosciences, Radboudumc, 2Donders Institute for Brain, Cognition and Behaviour, Radboud University, 3Department of Human Genetics, Radboudumc, 4Department of Molecular Developmental Biology, Radboud University
* These authors contributed equally

챕터

요약
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Vi ændre og gennemføre en tidligere offentliggjort protokol beskriver hurtig, reproducerbar og effektiv differentiering af humane inducerede pluripotente stamceller (hiPSCs) i excitatoriske kortikale neuroner 12. Specifikt vores modifikation giver mulighed for kontrol af neuronal celletæthed og brug på mikroelektrode-arrays til at måle elektrofysiologiske egenskaber på netværksniveau.

Tags

Developmental Biology inducerede pluripotente stamceller neuronal differentiering mikro-elektrodesæt lentiviral transduktion astrocyt isolation neuronal netværk
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter