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Biochemistry

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JoVE Journal Biochemistry
Muestra mejorada Multiplexing de tejidos usando combinada Precursor isotópico etiquetado y isobárico Tagging (cPILOT)
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Journal (Biochemistry)
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Journal (Biochemistry)
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Muestra mejorada Multiplexing de tejidos usando combinada Precursor isotópico etiquetado y isobárico Tagging (cPILOT)

Article DOI: 10.3791/55406-v • 09:06 min • May 1st, 2017
May 1st, 2017 •
1, 1, 2, 3, 1
1Department of Chemistry, University of Pittsburgh, 2SGS North America Inc., 3Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science, Janssen Research and Development

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Combinado precursor de marcaje isotópico y etiquetado isobárico (cPILOT) es una estrategia proteómica cuantitativa que mejora las capacidades de multiplexación de muestra de las etiquetas isobáricas. Este protocolo describe la aplicación de cPILOT a los tejidos de los controles de modelo de ratón de la enfermedad y de tipo salvaje de un Alzheimer.

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Bioquímica No. 123 proteómica cuantitativa cPILOT TMT marcaje isotópico y etiquetado isobárico multiplexación los tejidos
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