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Journal / Genetics / Vurdering av DNA forurensning i RNA eksempler…
JoVE Journal
Genetics

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Vurdering av DNA forurensning i RNA eksempler basert på Ribosomal DNA

Article DOI: 10.3791/55451-v • 13:16 min • January 22nd, 2018
January 22nd, 2018 •
1, 1, 2, 3, 4
1Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), 2Department of Industrial and Environmental Biotechnology, National Institute of Genetics Engineering and Biotechnology (NIGEB), 3Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences & Natural Resources, 4RG Abiotic Stress Genomics/ RG Heterosis, Department Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

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Her presenterer vi en protokoll for sporing genomisk DNA (gDNA) forurensning i RNA prøver. Presentert metoden benytter primere bestemt intern transkribert spacer regionen (ITS) ribosomal DNA (rDNA) gener. Metoden er egnet for pålitelig og følsom oppdagelsen av DNA forurensning i de fleste eukaryoter og prokaryoter.

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Genetikk problemet 131 qPCR RT-qPCR gene expression analyse DNA forurensning analysen ribosomal DNA (rDNA) ribosomal RNA (rRNA) Poaceae ifølge eukaryoter universell primer
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