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Genetics

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JoVE Journal Genetics
Retrospektiv MicroRNA sekvensering
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Retrospektiv MicroRNA sekvensering: Komplementære DNA biblioteket forberedelse protokollen bruker Formalin-fast parafin-embedded RNA prøver

Article DOI: 10.3791/57471-v • 12:24 min • May 5th, 2018
May 5th, 2018 •
1,2,3, 1,2, 3, 4
1Department of Research, Hackensack University Medical Center, 2Department of Medical Sciences, Seton Hall University, 3Department of Epidemiology and Population Health, Albert Einstein College of Medicine, 4Department of Nephrology and Hypertension, Hadassah - Hebrew University Medical Center

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Formalin-fast parafin-embedded prøver representerer en verdifull kilde til molekylær biomarkers menneskelige sykdommer. Her presenterer vi en laboratorie-baserte cDNA biblioteket forberedelse protokoll, opprinnelig designet med fersk frosne RNA og optimalisert for analyse av arkiverte microRNAs fra vev lagret opp til 35 år.

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Genetikk problemet 135 Formalin-fast parafin-innebygd FFPE RNA degradert RNA microRNA neste generasjons sekvensering cDNA biblioteket kvantifisering PCR forsterkning T4 RNA hemorroider siden degradert RNA
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