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Neuroscience

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JoVE Journal Neuroscience
Single-Cell Electroporation på tværs af forskellige organotypiske Slice Kultur Af Mouse Hippocampal Excitatory og klasse-specifikke hæmmende neuroner
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Single-Cell Electroporation på tværs af forskellige organotypiske Slice Kultur Af Mouse Hippocampal Excitatory og klasse-specifikke hæmmende neuroner

Article DOI: 10.3791/61662-v • 06:21 min • October 6th, 2020
October 6th, 2020 •
*1,2, *1,3, 1
1Brudnick Neuropsychiatric Research Institute, Department of Neurobiology, University of Massachusetts Medical School, 2Interdisciplinary Graduate Program, University of Massachusetts Medical School, 3UMMS MD/PhD Program, University of Massachusetts Medical School
* These authors contributed equally

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요약
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Præsenteret her er en protokol for enkelt-celle elektroporation, der kan levere gener i både excitatoriske og hæmmende neuroner på tværs af en række in vitro hippocampal skive kultur aldre. Vores tilgang giver præcis og effektiv ekspression af gener i individuelle celler, som kan bruges til at undersøge celle-autonome og intercellulære funktioner.

Tags

Neurovidenskab enkeltcellet elektroporation genlevering neuron interneuron elektrofysiologi hippocampus organotypic skive kultur mus
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