JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Department of Molecular Physiology and Biophysics,University of Virginia School of Medicine, 2Robert M. Berne Cardiovascular Research Center,University of Virginia School of Medicine, 3Department of Hematology,St. Jude Children’s Research Hospital, 4Research Histology Core,University of Virginia School of Medicine
Chapters
- 00:05Title
- 00:49Plating Cells for Vascular Cell co-culture
- 04:14Harvesting Fractions from the VCCC
- 07:21Harvesting and Embedding the VCCC for Transverse Immunofluorescence
- 09:11Results: Myoendothelial Junction Formation in Vascular Cell Co-cultures
- 10:18Conclusion
Se describe un modelo de cultivo celular de las arterias de resistencia, lo que permite la disección de la señalización de vías en el endotelio, músculo liso, o entre el endotelio y músculo liso (el cruce de myoendothelial). Puede utilizar la aplicación selectiva de agonistas o aislamiento de la proteína, microscopia electrónica o inmunofluorescencia usando este modelo de cultura de célula.
