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In Vivo Detectie en analyse van Rb eiwit SUMOylation in menselijke cellen
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In Vivo Detectie en analyse van Rb eiwit SUMOylation in menselijke cellen

Article DOI: 10.3791/56096-v • 09:41 min • November 2nd, 2017
November 2nd, 2017 •
*1,2, *1,2, 1,2, 1,2
1Department of Ophthalmology, Eye and ENT Hospital of Fudan University, 2Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University
* These authors contributed equally

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Kleine ubiquitin-gerelateerde modifier (SUMO) familie eiwitten zijn geconjugeerd lysine residuen van doel eiwitten voor het regelen van diverse cellulaire processen. Dit witboek beschrijft een protocol voor de detectie van Retinoblastoom (Rb) eiwit SUMOylation onder endogene en exogene voorwaarden in menselijke cellen.

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Moleculaire biologie kwestie 129 eiwit Retinoblastoom (Rb) posttranslationele modificatie (PTM) kleine ubiquitin-gerelateerde modifier (SUMO) celcyclus HKE293 cellen
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