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Stroom Cytometry gebaseerde Drug Screening systeem voor de identificatie van kleine moleculen die bevordering van celdifferentiatie van Glioblastoma stamcellen

Article DOI: 10.3791/56176-v • 10:28 min • January 10th, 2018
January 10th, 2018 •
1, 1, 2, 1,3, 1
1Department of Neurological Surgery, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine, 2Department of Pathology, Johns Hopkins University, School of Medicine, 3Department of Neurological Surgery, University Hospital-Case Medical Center, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine

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Een efficiënte screening-protocol wordt gepresenteerd voor de identificatie van kleine moleculen ter bevordering van astroglial differentiatie in glioblastoma stamcellen (GSCs). De bepaling is gebaseerd op een stamcel differentiatie verslaggever waarbij de expressie van de verbeterde GFP (eGFP) wordt gedreven door de menselijke promotor van het algemeen kaderakkoord voor vrede.

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Kankerbiologie kwestie 131 stamcellen Glioma Astrocytic differentiatie Lentivirus verslaggever systeem Drug scherm differentiatie therapie Acetyl Choline Calcium signalering
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