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Fysiologisch patiënt afgeleide 3D-Spheroids voor anti-neoplastische geneesmiddelen screening om kanker stamcellen te targeten

Article DOI: 10.3791/59696-v • 10:03 min • July 5th, 2019
July 5th, 2019 •
*1, *1, *1, *2, *1, *2, *2, 1,2,3,4
1Department of Biomedical Engineering, University of Michigan, 2Department of Materials Science and Engineering, University of Michigan, 3Rogel Cancer Center, School of Medicine, University of Michigan, 4Macromolecular Science and Engineering, University of Michigan
* These authors contributed equally

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Dit protocol beschrijft het genereren van door patiënten afgeleide spheroïden en downstreamanalyses, waaronder de kwantificering van proliferatie, cytotoxiciteits testen, Flowcytometrie, immunofluorescentie kleuring en confocale beeldvorming, om de geneesmiddelen te beoordelen potentiële kandidaten als anti-neoplastische Therapeutics. Dit protocol ondersteunt precisie geneeskunde met identificatie van specifieke geneesmiddelen voor elke patiënt en stadium van de ziekte.

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Kankeronderzoek probleem 149 patiënt afgeleid kanker stamcellen drug screening hoge doorvoer hangende druppel smeerïden precisie geneeskunde
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