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Bioengineering

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Langfristige Live Imaging-Gerät für verbesserte experimentelle Manipulation von Zebrafischlarven

Article DOI: 10.3791/56340-v • 14:05 min • October 27th, 2017
October 27th, 2017 •
*1,2, *3, 2, 2, 4, 5,6, 1,2,3
1Department of Biomedical Engineering, University of Wisconsin-Madison, 2Morgridge Institute for Research, University of Wisconsin-Madison, 3Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, 4Cellular and Molecular Pathology Graduate Program, University of Wisconsin-Madison, 5Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 6Department of Pediatrics, University of Wisconsin-Madison
* These authors contributed equally

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Dieses Manuskript beschreibt die zWEDGI (Zebrafisch daraus und verlockende Falle Gerät für Wachstum und Imaging), die ist ein unterteilte Gerät zu orientieren und zurückhalten Zebrafischlarven. Das Design erlaubt Schweif Durchtrennung und langfristige Sammlung von hochauflösender Fluoreszenz-Mikroskopie-Bildern der Wundheilung und Regeneration.

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Biotechnik Ausgabe 128 Wundheilung Zebrafisch multiphoton Mikroskopie Geräteabbilderstellung Zurückhaltung Larven langfristige
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