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Langsiktig Live bildeenheten forbedret eksperimentelle manipulering av sebrafisk larver

Article DOI: 10.3791/56340-v • 14:05 min • October 27th, 2017
October 27th, 2017 •
*1,2, *3, 2, 2, 4, 5,6, 1,2,3
1Department of Biomedical Engineering, University of Wisconsin-Madison, 2Morgridge Institute for Research, University of Wisconsin-Madison, 3Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, 4Cellular and Molecular Pathology Graduate Program, University of Wisconsin-Madison, 5Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 6Department of Pediatrics, University of Wisconsin-Madison
* These authors contributed equally

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Dette manuskriptet beskriver zWEDGI (sebrafisk Wounding og Entrapment enhet for vekst og bildebehandling), som er en compartmentalized enhet konstruert for å orientere og holde sebrafisk larver. Design tillater hale transection og langsiktig samling av høyoppløselige fluorescerende mikroskopi bilder av sårheling og gjenfødelse.

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Bioteknologi problemet 128 sårheling sebrafisk multiphoton mikroskopi imaging tilbakeholdenhet enhet larvene langsiktig
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