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Bioengineering

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JoVE Journal Bioengineering
Verbeterd 3D Hydrogel culturen van primaire gliacellen voor In Vitro modellering van Neuroinflammation
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Verbeterd 3D Hydrogel culturen van primaire gliacellen voor In Vitro modellering van Neuroinflammation

Article DOI: 10.3791/56615-v • 09:19 min • December 8th, 2017
December 8th, 2017 •
1,2, 1,2, 1,3, 1,2,4, 3, 1,2,5
1Department of Psychiatry, University of Alberta, 2Alberta Innovates-Health Solutions Interdisciplinary Team in Smart Neural Prostheses (Project SMART), University of Alberta, 3Department of Chemical and Materials Engineering, University of Alberta, 4Division of Physical Medicine and Rehabilitation, University of Alberta, 5Centre for Neuroscience, University of Alberta

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Hierin presenteren wij een protocol voor de 3D cultuur van rat hersenen-afgeleide glia cellen, met inbegrip van astrocyten, oligodendrocyten en microglia. We tonen primaire celculturen, methacrylated hyaluronzuur (HAMA) hydrogel synthese, HAMAphoto-polymerisatie en cel inkapseling en monster verwerking voor confocale en scanning elektronen microscopische beeldvorming.

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Bioengineering kwestie 130 3D-celkweek hydrogel primaire glia Astrocyt microglia Oligodendrocyt neuroinflammation hyaluronzuur HAMA
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