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Mejora 3D hidrogel culturas de células Glial primarias para In Vitro modelado de neuroinflamación

Article DOI: 10.3791/56615-v • 09:19 min • December 8th, 2017
December 8th, 2017 •
1,2, 1,2, 1,3, 1,2,4, 3, 1,2,5
1Department of Psychiatry, University of Alberta, 2Alberta Innovates-Health Solutions Interdisciplinary Team in Smart Neural Prostheses (Project SMART), University of Alberta, 3Department of Chemical and Materials Engineering, University of Alberta, 4Division of Physical Medicine and Rehabilitation, University of Alberta, 5Centre for Neuroscience, University of Alberta

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Adjunto, presentamos un protocolo para el cultivo 3D de rata células glia derivado del cerebro, incluyendo astrocitos, microglia y oligodendrocitos. Demostramos cultivo celular primario, síntesis de hidrogel desarrolla el ácido hialurónico (HAMA), encapsulación HAMAphoto-polimerización y de la célula y muestra de procesamiento para la proyección de imagen microscópica electrónica de barrido y confocal.

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Bioingeniería número 130 cultivo celular 3D hidrogel glia primaria astrositos microglía oligodendrocyte neuroinflamación ácido hialurónico HAMA
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