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Isolement et culture des progéniteurs neurones suivie par immunoprécipitation de la chromatine de marque d’Histone 3 Lysine 79 MBD

Article DOI: 10.3791/56631-v • 10:09 min • January 26th, 2018
January 26th, 2018 •
1,2, 1, 1, 1, 1
1Institute for Anatomy and Cell Biology, Department of Molecular Embryology, Faculty of Medicine, University of Freiburg, 2Faculty of Biology, University of Freiburg

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Nous présentons une méthode efficace et reproductible pour isoler et de la culture des cellules progénitrices neurales de tissus du cerveau embryonnaire et postnatale pour l’immunoprécipitation de la chromatine (ChIP) de l’histone 3 lysine 79 MBD (H3K79me2) - une marque d’histone agrave la domaine globulaire de l’histone 3.

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Neurosciences numéro 131 culture cellulaire cérébelleux culture de cellules corticales développement du cortex développement cérébelleux méthylation de l’H3K79 DOT1L puce inhibition de la DOT1L
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