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Neuroscience

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JoVE Journal Neuroscience
Isolierung und Kultivierung von neuronalen Vorläuferzellen gefolgt von Chromatin Immunopräzipitation Histon 3 Lysin 79 Dimethylation Marke
Induction of the Murine Subarachnoid Hemorrhage Model and Brain Slice Preparation for Fluorescent Imaging (Video) | JoVE
Journal (Neuroscience)
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Intravital Imaging of Axonal Interactions with Microglia and Macrophages in a Mouse Dorsal Column Crush Injury
Journal (Neuroscience)
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Isolierung und Kultivierung von neuronalen Vorläuferzellen gefolgt von Chromatin Immunopräzipitation Histon 3 Lysin 79 Dimethylation Marke

Article DOI: 10.3791/56631-v • 10:09 min • January 26th, 2018
January 26th, 2018 •
1,2, 1, 1, 1, 1
1Institute for Anatomy and Cell Biology, Department of Molecular Embryology, Faculty of Medicine, University of Freiburg, 2Faculty of Biology, University of Freiburg

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Wir präsentieren Ihnen eine effektive und reproduzierbare Methode zum isolieren und Kultur neurale Vorläuferzellen aus embryonalen und postnatale Hirngewebe für Chromatin Immunopräzipitation (ChIP) von Histon 3 Lysin 79 Dimethylation (H3K79me2) - eine Histon-Markierung befindet sich innerhalb der globulären Domäne des Histon 3.

Tags

Neurowissenschaften Ausgabe 131 zerebelläre Zellkultur kortikalen Zellkulturen Cortex Entwicklung zerebelläre Entwicklung H3K79 Methylierung DOT1L ChIP DOT1L Hemmung
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