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Journal / Immunology and Infection / Visualisera den aktin och mikrotubuli…
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Immunology and Infection

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Visualisera den aktin och mikrotubuli Cytoskeletons på B-cellen immun synapsen använder stimulerad Emission utarmning (STED) mikroskopi
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Visualisera den aktin och mikrotubuli Cytoskeletons på B-cellen immun synapsen använder stimulerad Emission utarmning (STED) mikroskopi

Article DOI: 10.3791/57028-v • 11:00 min • April 9th, 2018
April 9th, 2018 •
1, 1, 1
1Department of Microbiology and Immunology, University of British Columbia

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Vi presenterar ett protokoll för att använda STED mikroskopi samtidigt bilden aktin strukturer, mikrotubuli och mikrotubuli plus-end bindande proteiner i B-celler som har spridit sig på coverslips belagd med antikroppar till B-cells receptor, en modell för den inledande fasen immun synaps bildas.

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Immunologi och infektion fråga 134 immunologi B-celler immun synaps aktin mikrotubuli mikrotubuli-organisera center (MTOC) plus-end spårning proteiner super-resolution mikroskopi stimulerad emission utarmning (STED) mikroskopi
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