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Journal / Immunology and Infection / Ett lättköpt protokoll att generera…
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Ett lättköpt protokoll att generera anläggningsvis acetylerade proteiner i Escherichia Coli
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Journal (Immunology and Infection)
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Ett lättköpt protokoll att generera anläggningsvis acetylerade proteiner i Escherichia Coli

Article DOI: 10.3791/57061-v • 11:09 min • December 9th, 2017
December 9th, 2017 •
1,2, 3, 4, 1, 1,2
1Department of Chemistry and Biochemistry, University of Arkansas, 2Cell and Molecular Biology Program, University of Arkansas, 3Department of Biological Sciences, University of Arkansas, 4Fayetteville High School

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Genetiska koden expansion fungerar som ett kraftfullt verktyg för att studera en mängd biologiska processer, inklusive protein acetylering. Här visar vi ett lättköpt protokoll att utnyttja denna teknik för att generera homogeneously acetylerade proteiner på specifika platser i Escherichia coli celler.

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Immunologi fråga 130 Protein acetylering posttranslationella modifieringen genetiska koden expansion noncanonical aminosyror malate dehydrogenas syntetisk biologi
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