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Journal / Genetics / Chromatine Immunoprecipitation (ChIP) van Histone …
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Genetics

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Chromatine Immunoprecipitation (ChIP) van Histone modificaties van Saccharomyces cerevisiae
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Chromatine Immunoprecipitation (ChIP) van Histone modificaties van Saccharomyces cerevisiae

Article DOI: 10.3791/57080-v • 11:07 min • December 29th, 2017
December 29th, 2017 •
1, 1, 1, 1
1Department of Biological Sciences, University of Maryland

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Hier beschrijven we een protocol voor het chromatine immunoprecipitation van gemodificeerde histonen uit de ontluikende gist Saccharomyces cerevisiae. Immunoprecipitated DNA wordt vervolgens gebruikt voor kwantitatieve PCR te ondervragen de overvloed en lokalisatie van Histon posttranslationele modificaties in het genoom.

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Genetica kwestie 130 epigenetica chromatine histone modificaties methylering acetylation chromatine immunoprecipitation gist
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