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Chromatin Immunopräzipitation (ChIP) Histon-Modifikationen von Saccharomyces cerevisiae

Article DOI: 10.3791/57080-v • 11:07 min • December 29th, 2017
December 29th, 2017 •
1, 1, 1, 1
1Department of Biological Sciences, University of Maryland

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Hier beschreiben wir ein Protokoll für Chromatin Immunopräzipitation der geänderten Histone aus der angehende Hefe Saccharomyces Cerevisiae. Immunoprecipitated DNA wird anschließend für die quantitative PCR verwendet, um die Fülle und die Lokalisierung von Histon post-translationalen Modifikationen während des Genoms zu befragen.

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Genetik Ausgabe 130 Epigenetik Chromatin Histon-Modifikationen Methylierung Acetylierung Chromatin Immunopräzipitation Hefe
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