Journal
/
발생학
/
Live Cell Imaging of Chromosome Segregation During Mitosis
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Live Cell Imaging of Chromosome Segregation During Mitosis

Live Cell Imaging of Chromosome Segregation During Mitosis

DOI:
10.3791/57389-v

•

06:39 min

•

March 14, 2018

•

, ,
1Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research,US Food and Drug Administration, 2Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research,US Food and Drug Administration

Chapters

  • 00:04Title
  • 01:04Labeling Cells
  • 02:21Visualization of Lagging Chromosomes
  • 04:45Results: Nocodazole-treated MEFs Lacking PP2A-B56 Undergo Chromosomal Mis-segregation
  • 05:29Conclusion

Summary

자동 번역

자동 번역

This protocol describes an easy and convenient method to label and visualize live chromosomes in mitotic cells using Histone2B-GFP BacMam 2.0 label and a spinning disc confocal microscopy system.

Tags

Live Cell ImagingChromosome SegregationMitosisHistone 2B-GFP BacMam 2.0Spinning Disc Confocal MicroscopyMouse Embryonic FibroblastsNocodazoleCell SynchronizationLive Chromosome VisualizationEnvironmental ChamberOil Immersion Objective

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Live Confocal Imaging of Developing Arabidopsis Flowers

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

Live Imaging of Mouse Secondary Palate Fusion

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem

Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos

Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad

Read Article