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JoVE Journal
Developmental Biology

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Live Cell Imaging of Chromosome Segregation During Mitosis

Live Cell Imaging of Chromosome Segregation During Mitosis

Article DOI: 10.3791/57389 06:39 min March 14th, 2018
March 14th, 2018



This protocol describes an easy and convenient method to label and visualize live chromosomes in mitotic cells using Histone2B-GFP BacMam 2.0 label and a spinning disc confocal microscopy system.



Live Cell Imaging Chromosome Segregation Mitosis Histone 2B-GFP BacMam 2.0 Label Spinning Disc Confocal Microscopy Genetic Modifications Drug Treatments Easy And Convenient Method Doctor Kazuyo Takeda Microscopy And Imaging Core Facility Division Of Viral Products Mouse Embryonic Fibroblasts (MEFs) Two-well Chambered Cover Glass Synchronization G0/G1 Phase DMEM/F12 Growth Medium Nocodazole CL-HB Particles
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