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Laser fange Microdissection af yderst rene trabekelværket fra mus øjne for gen Expression analyse

Article DOI: 10.3791/57576-v • 13:47 min • June 3rd, 2018
June 3rd, 2018 •
*1, *2, *1, 2, 2, 2,3, 2,4, 1
1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research, National Institute of Environmental Health Sciences, NIH, 2Cellular and Molecular Pathology Branch, Division of the National Toxicology Program, National Institute of Environmental Health Sciences, NIH, 3Integrated Laboratory Systems Inc., 4Experimental Pathology Laboratories Inc.
* These authors contributed equally

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Her, beskriver vi en protokol for en reproducerbar laser fange microdissection (LCM) til isolering af trabekelværket (TM) for downstream RNA analyse. Evnen til at analysere ændringer i genekspression i TM vil hjælpe med at forstå de underliggende molekylære mekanismer af TM-okulær sygdomme.

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Biologi sag 136 glaukom trabekelværket laser fange microdissection laser microdissection RNA isolering Gen-ekspression LCM LMD
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