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Developmental Biology

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JoVE Journal Developmental Biology
Efficiënte differentiatie van menselijke pluripotente stamcellen in levercellen
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Efficiënte differentiatie van menselijke pluripotente stamcellen in levercellen

Article DOI: 10.3791/58975-v • 07:37 min • June 11th, 2019
June 11th, 2019 •
1,2, 1, 1
1Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute, Stanford University School of Medicine, 2Department of Developmental Biology, Stanford University School of Medicine

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Dit protocol Details een monolayer, serum-vrije methode voor het efficiënt genereren van hepatocyte-achtige cellen van menselijke pluripotente stamcellen (hPSCs) in 18 dagen. Dit omvat zes stappen als hpscs sequentieel differentiëren in tussenliggende celtypen zoals de primitieve streak, definitieve endoderm, posterieure foregut en lever Bud voor ouders voor het vormen van hepatocyte-achtige cellen.

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Ontwikkelingsbiologie uitgave 148 humane pluripotente stamcel efficiënt differentiatie endoderm lever voorlopercellen hepatocyten
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