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Journal / Developmental Biology / Analys av hjärtats kammare utveckling under musen …
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Developmental Biology

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Analys av hjärtats kammare utveckling under musen embryogenes använder hela Mount Epifluorescence

Article DOI: 10.3791/59413-v • 06:27 min • April 17th, 2019
April 17th, 2019 •
1,2,3, 1,2,3
1Department of Medicine, Division of Cardiovascular Medicine, Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology, Vanderbilt University, 3Vanderbilt Center for Stem Cell Biology, Vanderbilt University

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Vi presenterar de protokoll för att undersöka mus hjärtat utveckling med hela mount epifluorescerande mikroskopi på musembryon dissekeras från ventrikulära specifika MLC-2v-tdTomato reporter inpressning möss. Denna metod tillåter oss att direkt visualisera varje skede av ventrikulära bildandet under musen hjärtat utveckling utan arbetsintensiva histochemical metoder.

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Utvecklingsbiologi fråga 146 hjärtat Embryo ventrikeln kammare hela berget och Epifluorescence
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