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Journal / Biochemistry / Lokalisierung von SUMO-modifizierten Proteinen…
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Biochemistry

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Lokalisierung von SUMO-modifizierten Proteinen mit fluoreszierenden Sumo-Fallen Proteinen

Article DOI: 10.3791/59576-v • 06:23 min • April 27th, 2019
April 27th, 2019 •
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1Integrated Science Center, Biology Department, College of William & Mary

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SUMO ist ein essentielles und hoch konserviertes, kleines, ubiquitin-ähnliches Modifikatorprotein. In diesem Protokoll beschreiben wir die Verwendung eines stresstoleranten rekombinanten, rekombinanten SUMO-Fantifen-Proteins (kmUTAG) zur Visualisierung von nativen, nicht markierten SUMO-Konjugaten und deren Lokalisierung in einer Vielzahl von Zelltypen.

Tags

Biochemie Ausgabe 146 SUMO Smt3 Smo-1 Sumoylierung C. elegans PNT2 Baufeuchte UTAG SUMO-Fallen UD
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