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Biochemistry

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Anwendung der Biolayer-Interferometrie (BLI) zur Untersuchung von Protein-Protein-Wechselwirkungen bei der Transkription

Article DOI: 10.3791/59687-v • 07:18 min • July 26th, 2019
July 26th, 2019 •
1,2, 3, 1,2
1Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers University, 2Graduate Program in Physiology and Integrative Biology, School of Graduate Studies, Rutgers University, 3Department of Plant Biology, School of Environmental and Biological, Rutgers University

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Wechselwirkungen von Transkriptionsfaktoren (TFs) mit der RNA-Polymerase werden in der Regel mit Pulldown-Assays untersucht. Wir wenden eine Biolayer Interferometry (BLI) Technologie an, um die Wechselwirkung von GrgA mit der Chlamydien-RNA-Polymerase zu charakterisieren. Im Vergleich zu Pulldown-Assays erkennt BLI Echtzeit-Assoziation und Dissoziation, bietet eine höhere Empfindlichkeit und ist sehr quantitativ.

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In diesem Monat in JoVE Ausgabe 149 Biolayer-Interferometrie Chlamydien CT504 TC0791 GrgA Protein-Protein-Interaktion Transkriptionsfaktoren Transkriptionsregulation
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