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Journal / Immunology and Infection / 質量分析による3つの DNA…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
質量分析による3つの DNA 病変の定量化と、周囲の微細粒子状物質に曝露したマウスの組織におけるそのレベルの評価
Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy
Journal (Immunology and Infection)
Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy
Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood
Journal (Immunology and Infection)
Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood
 
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質量分析による3つの DNA 病変の定量化と、周囲の微細粒子状物質に曝露したマウスの組織におけるそのレベルの評価

Article DOI: 10.3791/59734-v • 12:15 min • May 29th, 2019
May 29th, 2019 •
*1,2, *1, 3, 3, 3,4, 5, 5, 1
1Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, 2Departamento de Farmacociências, Universidade Federal de Ciências da Saúde de Porto Alegre, 3Laboratório de Poluição Atmosfêrica Experimental - LIM05, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, 4Instituto de Estudos Avançados, Universidade de São Paulo, 5Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo
* These authors contributed equally

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ここでは、病変の高感度かつ正確な定量のための方法 8-オキソ-7, 8-ジヒドロ-2'-デオキシグアノシン (8-oxodGuo), 1,n6-etheno-2'-デオキシアデノシン (1,n6-茶道) および 1,n2-etheno-2'-デオキシグアノシン (1,N2-dGuo) DNA で.この方法は、曝露された A/J マウスの組織 (肺、肝臓および腎臓) における周囲微細粒子状物質 (PM2.5) の影響の評価に適用した。

Tags

免疫学および感染症、問題147、質量分析、DNA 付加物、DNA 酸化、遺伝毒性、酸化ストレス、微粒子物質
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