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Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage

Article DOI: 10.3791/59924-v • 12:59 min • February 15th, 2020
February 15th, 2020 •
*1, *1, *1, 1, 1, 1
1Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangaluru-575018, Karnataka, India
* These authors contributed equally

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This protocol demonstrates the simultaneous detection of reactive oxygen species (ROS), live cells, and dead cells in live primary cultures from mouse ocular surface cells. 2',7'-Dichlorofluoresceindiacetate, propidium iodide, and Hoechst staining are used to assess the ROS, dead cells, and live cells, respectively, followed by imaging and analysis.

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Oxidative Damage Primary Mouse Ocular Surface Cells Stem Cells Ultraviolet-C (UV-C) Damage ROS (reactive Oxygen Species) Detection Technique Live Cell Permeability DCFDA Dye Green Fluorescence Propidium Iodide Dye Red Fluorescence Hoechst Dye Blue Nuclear Stain
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