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Journal / Cancer Research / Kultur, Manipulation und orthotopische…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Kultur, Manipulation und orthotopische Transplantation von Mausblase Tumororganoiden
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Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies
Journal (Cancer Research)
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Kultur, Manipulation und orthotopische Transplantation von Mausblase Tumororganoiden

Article DOI: 10.3791/60469-v • 08:43 min • January 31st, 2020
January 31st, 2020 •
*1, *1, *1, 1
1Department of Life Sciences, Pohang University of Science and Technology
* These authors contributed equally

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Dieses Protokoll enthält detaillierte experimentelle Schritte zur Etablierung einer dreidimensionalen In-vitro-Kultur von Blasentumororganoiden, die aus krebserregendem murinen Blasenkrebs gewonnen werden. Kulturmethoden wie Passaging, Gentechnik und orthotopische Transplantation von Tumororganoiden werden beschrieben.

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Krebsforschung Ausgabe 155 Tumororganoide 3D-Kultur Blasentumor invasives urotheliale Karzinom lentivirale Transduktion orthotopische Transplantation
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