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Cancer Research

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JoVE Journal Cancer Research
Culture, Manipulation et Transplantation orthotopique d'organoïdes tumoraux de la vessie de souris
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Culture, Manipulation et Transplantation orthotopique d'organoïdes tumoraux de la vessie de souris

Article DOI: 10.3791/60469-v • 08:43 min • January 31st, 2020
January 31st, 2020 •
*1, *1, *1, 1
1Department of Life Sciences, Pohang University of Science and Technology
* These authors contributed equally

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Ce protocole fournit des étapes expérimentales détaillées pour établir une culture in vitro tridimensionnelle des organoïdes de tumeur de réservoir souple dérivés du cancer de réservoir souple cancérogène-induit. Des méthodes de culture comprenant le passage, le génie génétique, et la transplantation orthotopic des organoïdes de tumeur sont décrites.

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Recherche sur le cancer numéro 155 organoïdes tumoraux culture 3D tumeur de la vessie carcinome urothélial invasif transduction lentivirale transplantation orthotopic
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