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Immunology and Infection

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TurboID-Based Proximity Labeling voor in Planta Identificatie van Protein-Protein Interaction Networks

Article DOI: 10.3791/60728-v • 07:02 min • May 17th, 2020
May 17th, 2020 •
*1, *2,3, 1, 1, 2, 2,3
1State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, 2Department of Plant Biology, College of Biological Sciences, University of California, Davis, 3Genome Center, College of Biological Sciences, University of California, Davis
* These authors contributed equally

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Hier beschreven is een nabijheid etikettering methode voor de identificatie van interactie partners van de TIR domein van de NLR immuunreceptor in Nicotiana benthamiana bladweefsel. Ook is een gedetailleerd protocol voor de identificatie van interacties tussen andere eiwitten van belang met behulp van deze techniek in Nicotiana en andere plantensoorten.

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Immunologie en infectie nummer 159 proximity labeling TurboID eiwitinteracties ontzouten kwantificering zuivering TIR
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