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Journal / Biology / Agrobacterium-Gemedeerde onrijpe…
JoVE Journal
Biology

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Agrobacterium-Gemedeerde onrijpe embryotransformatie van recalcitrante maïsinteeltlijnen met behulp van morfogene genen

Article DOI: 10.3791/60782-v • 10:28 min • February 14th, 2020
February 14th, 2020 •
1,2, 1,3,4, 1,3, 1,3,5, 2, 2, 1,3
1Department of Agronomy, Iowa State University, 2Department of Applied Science and Technology, Corteva Agriscience, 3Crop Bioengineering Center, Iowa State University, 4Interdepartmental Plant Biology Major, Iowa State University, 5Interdepartmental Genetics and Genomics Major, Iowa State University

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Plant morfogene genen kunnen worden gebruikt om de genetische transformatie van recalcitrante genotypes te verbeteren. Hier beschreven is een Agrobacterium-gemedieerde genetische transformatie (QuickCorn) protocol voor drie belangrijke openbare maïs inteelt lijnen.

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Deze maand in JoVE Kwestie 156 Agrobacterium tumefaciens B73 Bbm genetische transformatie Mo17 QuickCorn transgenic W22 Wus2 Zea mays
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