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Developmental Biology

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Vorbereitung von Drosophila Larval und Pupal Hoden für die Analyse der Zellteilung in Leben, intaktes Gewebe
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Vorbereitung von Drosophila Larval und Pupal Hoden für die Analyse der Zellteilung in Leben, intaktes Gewebe

Article DOI: 10.3791/60961-v • 08:05 min • May 19th, 2020
May 19th, 2020 •
1, 1
1Department of Anatomy, Physiology, and Genetics, F. Edward Hébert School of Medicine, Uniformed Services University of the Health Sciences

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Das Ziel dieses Protokolls ist es, die Zellteilung im intakten Gewebe durch lebende und feste Zellmikroskopie mit Drosophila meiotischen Spermatozyten zu analysieren. Das Protokoll zeigt, wie ganze, intakte Hoden von Drosophila-Larven und frühen Pupas isoliert und wie man sie für die Mikroskopie verarbeitet und montiert.

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Entwicklungsbiologie Ausgabe 159 Drosophila Spermatozyten Hoden Mitose Meiose Zellteilung Mikrotubuli Spindel endoplasmatisches Retikulum
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