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Journal / Immunology and Infection / マウスB-1a細胞におけるCXCR4のレトロウイルス過剰発現と骨髄およびIgM産生への標的B-1a細…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
マウスB-1a細胞におけるCXCR4のレトロウイルス過剰発現と骨髄およびIgM産生への標的B-1a細胞の移行のための養子転移
Antibiotic Dereplication Using the Antibiotic Resistance Platform
Journal (Immunology and Infection)
Antibiotic Dereplication Using the Antibiotic Resistance Platform
Collection, Embedding, Sectioning, and Staining of the Mouse Submandibular Salivary Gland for Live Cell Imaging of Cell-Cell Interactions (Video) | JoVE
Journal (Immunology and Infection)
Collection, Embedding, Sectioning, and Staining of the Mouse Submandibular Salivary Gland for Live Cell Imaging of Cell-Cell Interactions (Video) | JoVE
 
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マウスB-1a細胞におけるCXCR4のレトロウイルス過剰発現と骨髄およびIgM産生への標的B-1a細胞の移行のための養子転移

Article DOI: 10.3791/61003-v • 08:22 min • May 31st, 2020
May 31st, 2020 •
1, 2, 2, 3, 2
1Department of Microbiology, Immunology, Cancer Biology, University of Virginia, 2Cardiovascular Research Center, University of Virginia, 3Beirne B. Carter Center for Immunology Research, University of Virginia

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ここでは、生体内B-1a細胞の移行および局在化を調べるマウスB-1a細胞のレトロウイルス過剰発現および導入導入の方法について説明する。このプロトコルは、ドナーB-1a細胞の局在化の定量化や、導入後のドナー細胞由来分泌因子の分析を含む、多様な下流機能アッセイに拡張することができる。

Tags

免疫学と感染症、問題159、養子転写、細胞移動、細胞局在、フローサイトメトリー、レトロウイルス伝達、B-1a細胞
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