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Journal / Immunology and Infection / CXCR4在Murine…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
CXCR4在Murine B-1a细胞上的逆转录病毒过度表达和靶向B-1a细胞向骨髓和IgM生产转移的采用转移
Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds
Journal (Immunology and Infection)
Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds
Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
Journal (Immunology and Infection)
Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
 
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CXCR4在Murine B-1a细胞上的逆转录病毒过度表达和靶向B-1a细胞向骨髓和IgM生产转移的采用转移

Article DOI: 10.3791/61003-v • 08:22 min • May 31st, 2020
May 31st, 2020 •
1, 2, 2, 3, 2
1Department of Microbiology, Immunology, Cancer Biology, University of Virginia, 2Cardiovascular Research Center, University of Virginia, 3Beirne B. Carter Center for Immunology Research, University of Virginia

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在这里，我们描述了一种抗逆转录病毒过度表达和采用迁移的鼠B-1a细胞的方法，以检查体内B-1a细胞的迁移和定位。该协议可扩展到不同的下游功能检测，包括定量供体B-1a细胞定位或分析供体细胞衍生分泌因子的收养后转移。

Tags

免疫学和感染，第159期，收养转移，细胞迁移，细胞定位，流动细胞学，逆转录病毒转导，B-1a细胞
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