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Obtention d’ostéocytes primaires par fractionnement calvariaire murin d’ostéocytes exprimant la GFP

Article DOI: 10.3791/61513-v • 07:22 min • June 2nd, 2020
June 2nd, 2020 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine, Tohoku University Graduate School of Dentistry

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Ce protocole décrit la dissection du calvaire néonatal de souris dmp1-topaze et l’isolement des ostéocytes exprimant la protéine fluorescente verte par digestion et fractionnement cellulaire, en plus de la préparation des ostéocytes pour le tri cellulaire activé par fluorescence (FACS).

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Rétraction numéro 160 ostéocytes primaires Dmp1 SOST GFP fractionnement FACS
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