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Journal / Biology / Agrobacterium-Transformación Genética Mediada,…
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Biology

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Agrobacterium-Transformación Genética Mediada, Producción Transgénica, y su Aplicación para el Estudio del Desarrollo Reproductivo Masculino en el Arroz
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Agrobacterium-Transformación Genética Mediada, Producción Transgénica, y su Aplicación para el Estudio del Desarrollo Reproductivo Masculino en el Arroz

Article DOI: 10.3791/61665-v • 07:43 min • October 6th, 2020
October 6th, 2020 •
*1,2, *1, 1, 3, 1,3
1Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 2College of Agronomy, Anhui Agricultural University, Heifei, China, 3School of Agriculture, Food and Wine, University of Adelaide
* These authors contributed equally

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Este trabajo describe el uso de la tecnología de edición del genoma CRISPR-Cas9 para eliminar el gen endógeno OsABCG15 seguido de un protocolo de transformación mediado por Agrobacteriummodificado para producir una línea estable de color masculino-estéril en el arroz.

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Este mes en JoVE Número 164 Arroz CRISPR-Cas9 Agrobacterium,callo cultivo de tejidos genotipo fenotipo antera polen
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