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Immunology and Infection

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Contactvrij co-kweekmodel voor de studie van innate immuuncelactivering tijdens respiratoire virusinfectie
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Contactvrij co-kweekmodel voor de studie van innate immuuncelactivering tijdens respiratoire virusinfectie

Article DOI: 10.3791/62115-v • 07:36 min • February 28th, 2021
February 28th, 2021 •
*1, *1, 1, 2, 3, 1,4, 1,4, 5, 2,6, 1,2,6, 1,6
1Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 3Haartman Institute, Medicum, University of Helsinki, 4Department of Otolaryngology, Head and Neck Surgery, National University Hospital, National University Health System, 5Agency for Science, Technology and Research (A*STAR), Singapore Immunology Network (SIgN), 6NUHS Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore
* These authors contributed equally

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Dit protocol beschrijft een onderzoek naar de vroege interacties tussen viraal geïnfecteerde neusepitheelcellen en aangeboren celactivatie. Individuele subsets van immuuncellen kunnen worden onderscheiden op basis van hun activering als reactie op virale infecties. Ze kunnen vervolgens verder worden onderzocht om hun effecten op vroege antivirale reacties te bepalen.

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Immunologie en infectie Co-cultuur menselijke nasale epitheelcellen aangeboren immuuncellen aangeboren T-cellen respiratoir virus Influenza cytokines flowcytometrie
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