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Cancer Research

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Modelo Biomimético Tridimensional In Vitro de Neuroblastoma Utilizando Arcabouços à Base de Colágeno
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Modelo Biomimético Tridimensional In Vitro de Neuroblastoma Utilizando Arcabouços à Base de Colágeno

Article DOI: 10.3791/62627-v • 07:48 min • July 9th, 2021
July 9th, 2021 •
*1,2,3, *1,2,3, 4, 3,5,6, 1,2,3,6,7
1Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 2School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 3Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 7Department of Chemistry, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin 2, Ireland, 5Trinity Centre for Bioengineering, Trinity College Dublin, Dublin, Ireland, 6Advanced Materials and Bioengineering Research Centre (AMBER), RCSI and TCD, Dublin, Ireland, 7National Children’s Research Centre, Our Lady's Children's Hospital Crumlin, Dublin, Ireland
* These authors contributed equally

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Este artigo lista as etapas necessárias para semear linhagens celulares de neuroblastoma em arcabouços tridimensionais à base de colágeno previamente descritos, manter o crescimento celular por um período de tempo pré-determinado e recuperar arcabouços para várias análises de crescimento e comportamento celular e aplicações a jusante, adaptáveis para satisfazer uma variedade de objetivos experimentais.

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Tridimensional In Vitro Modelo Biomimético Neuroblastoma Arcabouços à Base de Colágeno Câncer Pediátrico Tecido Tumoral Matriz Extracelular (MEC) Progressão da Doença Prognóstico do Paciente Resposta Terapêutica Linhagens Celulares de Neuroblastoma Nanohidroxiapatita (nHA) Glicosaminoglicanos (GAGs) Medula Óssea e Óssea Sítios Metastáticos Estrutura Porosa Fixação Celular Proliferação Migração Aglomerados Celulares Terapêutica Situação In Vivo Sistema de Cultura Baseado em Arcabouços Célula Bidimensional (2D) Cultura Quantificação de DNA Viabilidade Celular
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