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Biochemistry

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JoVE Journal Biochemistry
Het meten van de samenstelling van cd95-doodsopwekkend signaleringscomplex en verwerking van procaspase-8 in dit complex
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
Journal (Biochemistry)
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
Journal (Biochemistry)
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
 
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Het meten van de samenstelling van cd95-doodsopwekkend signaleringscomplex en verwerking van procaspase-8 in dit complex

Article DOI: 10.3791/62842-v • 07:17 min • August 2nd, 2021
August 2nd, 2021 •
1, 1
1Translational Inflammation Research, Center of Dynamic Systems, Otto von Guericke University Magdeburg

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Hier wordt een experimentele workflow gepresenteerd die de detectie van caspase-8-verwerking direct bij het death-inducerende signaleringscomplex (DISC) mogelijk maakt en de samenstelling van dit complex bepaalt. Deze methodologie heeft brede toepassingen, van het ontrafelen van de moleculaire mechanismen van celdoodroutes tot de dynamische modellering van apoptosenetwerken.

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Biochemie Nummer 174 CD95 DISC-vorming immunoprecipitatie caspase-8
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