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Journal / Biochemistry / Eiwitcomplex Affiniteit Capture uit Cryomilled…
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Biochemistry

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Eiwitcomplex Affiniteit Capture uit Cryomilled zoogdiercellen
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Eiwitcomplex Affiniteit Capture uit Cryomilled zoogdiercellen

Article DOI: 10.3791/54518-v • 10:37 min • December 9th, 2016
December 9th, 2016 •
1,2, 1, 1
1Laboratory of Cellular and Structural Biology, The Rockefeller University, 2Institute for Systems Genetics, Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine

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Hier beschrijven we protocollen bij zoogdiercellen door solid-state frezen verstoren bij een cryogene temperatuur, produceren een cel uittreksel uit de resulterende cel poeder, en te isoleren eiwitcomplexen van rente door affiniteitsinvangmiddel upon-antilichaam gekoppeld micron-schaal paramagnetische kralen.

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Biochemie affiniteitsinvangmiddel affiniteitszuivering immunoprecipitatie IP cryomilling cel ontwrichting eiwitcomplex zuivering
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