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Biochemistry

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JoVE Journal Biochemistry
Expression bactérienne et purification de la métalloprotéinase-3 à matrice humaine par chromatographie d’affinité
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High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)
 
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Expression bactérienne et purification de la métalloprotéinase-3 à matrice humaine par chromatographie d’affinité

Article DOI: 10.3791/63263-v • 07:32 min • March 30th, 2022
March 30th, 2022 •
*1, *1, 1
1Department of Chemical & Materials Engineering, University of Nevada, Reno
* These authors contributed equally

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La purification, la dialyse et l’activation de son étiquette sont utilisées pour augmenter les rendements de l’expression des protéines solubles du domaine catalytique de la métalloprotéinase-3 à matrice active chez les bactéries. Les fractions protéiques sont analysées via des gels SDS-PAGE.

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Biochimie numéro 181 Matrix metalloproteinase MMP-3cd Expression bactérienne Solubilisation et repliement des protéines humaines chez E. coli Purification des protéines d’affinité His-tag.
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