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Journal / Immunology and Infection / عزل براز الفئران وزرع…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
عزل براز الفئران وزرع الجراثيم
Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood
Journal (Immunology and Infection)
Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood
Increased Recovery Time and Decreased LPS Administration to Study the Vagus Nerve Stimulation Mechanisms in Limited Inflammatory Responses
Journal (Immunology and Infection)
Increased Recovery Time and Decreased LPS Administration to Study the Vagus Nerve Stimulation Mechanisms in Limited Inflammatory Responses
 
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عزل براز الفئران وزرع الجراثيم

Article DOI: 10.3791/64310-v • 07:32 min • May 26th, 2023
May 26th, 2023 •
1, 2,3, 2, 1
1Division of Clinical Pharmacology, Department of Medicine Vanderbilt University Medical Center and Department of Molecular Physiology and Biophysics, Vanderbilt University, 2Division of Pediatric Nephrology Department of Pediatrics, Vanderbilt University Medical Center, 3Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center

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الهدف هنا هو تحديد بروتوكول للتحقيق في آليات dysbiosis في أمراض القلب والأوعية الدموية. تناقش هذه الورقة كيفية جمع عينات براز الفئران وزرعها بشكل معقم ، وعزل الأمعاء ، واستخدام طريقة "Swiss-roll" ، تليها تقنيات التلوين المناعي لاستجواب التغيرات في الجهاز الهضمي.

Tags

علم المناعة والعدوى ، العدد 195 ، زرع الجراثيم البرازية ، لفة سويسرية ، القلب والأوعية الدموية
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